Longyue Liu

Human Liver Microsomes (HLM) are essential in the study of drug metabolism, providing an in vitro system to investigate the metabolic pathways and enzymatic processes that occur in the liver. These microsomes, derived from human liver cells, contain a rich mixture of drug-metabolizing enzymes, including the cytochrome P450 (CYP) family, which play a pivotal role in the oxidative metabolism of drugs. Understanding HLM drug metabolism is crucial for predicting drug clearance, potential drug-drug interactions, and the formation of active or toxic metabolites, thereby informing drug design and safety assessments. 

In my research, I utilize High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS) to analyze and quantify drug metabolites. HPLC offers high resolution and sensitivity for separating complex mixtures, while LC-MS combines this separation power with mass spectrometric detection, providing detailed structural information and precise quantification of metabolites. Together, these techniques allow for comprehensive profiling of metabolic pathways and the identification of metabolites with high specificity and accuracy, facilitating a deeper understanding of drug metabolism and its implications in pharmacokinetics and toxicology.