During development, coordinated signaling events genetically encode thousands of unique cell types along the body axis which contribute to the precisely organized neural circuits that shape nuanced behaviors. Disruption to these local networks by traumatic spinal cord injury or neurodegenerative diseases cause paralysis and autonomic deficits that are currently incurable. We seek to use human pluripotent stem cells to understand and develop strategies to regenerate region-specific neural circuits with the goal of advancing personalized therapeutics from the bench to the clinic.