Carolina Alves Feliciano
Infections by C. difficile depend on its infectious spore form germinating in the gut of susceptible individuals. Since spore germination depends on specific modifications made to the spore cell wall, determining how this process occurs in C. difficile could inform the development of strategies for preventing C. difficile disease transmission and recurrence. C. difficile uses a distinct mechanism, dependent on a protein complex, to modify the peptidoglycan cortex layer. I am using genetic and biochemical methods to understand the mechanism of cortex modification in C. difficile and compare it with other spore-forming bacteria.