Our research focuses on mineralized tissue – skeletal and craniofacial - development, homeostasis, disease and regeneration. Two main approaches are used: one using principles of tissue engineering (TE) for regenerative medicine and dentistry; and another using zebrafish as models for human mineralized tissue diseases. Our TE studies use post-natal dental stem cells harvested from extracted wisdom teeth and seeded onto biodegradable scaffolds, which are then implanted in in vivo animal models, to regenerate organized tooth crowns, periodontal tissues, and whole teeth. Using the developmental zebrafish model, we use a forward genetic mutagenesis approach combined with an in vivo mineralized tissue stain, to identify craniofacial and skeletal mutants. We believe that the synergistic nature of these combined approaches provide a unique opportunity to identify and functionally characterize genes regulating craniofacial and skeletal development and regeneration, and to test them in clinically relevant model systems.