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Graduate School of Biomedical Sciences

Recent Publications from the Isberg Lab

Fasciano AC, Dasanayake GS, Estes MK, Zachos NC, Breault DT, Isberg RR, Tan S, Mecsas J. 2021. Yersinia pseudotuberculosis YopE prevents uptake by M cells and instigates M cell extrusion in human ileal enteroid-derived monolayers. Gut Microbes. 13(1):1988390. doi: 10.1080/19490976.2021.1988390. Abstract

Choi WY, Kim S, Aurass P, Huo W, Creasey EA, Edwards M, Lowe M, Isberg RR. 2021. SdhA blocks disruption of the Legionella-containing vacuole by hijacking the OCRL phosphatase. Cell Rep. Nov 2;37(5):109894. doi: 10.1016/j.celrep.2021.109894. Abstract

Zhang M, McEwen JM, Sjoblom NM, Kotewicz KM, Isberg RR, Scheck RA. 2021. Members of the Legionella pneumophila Sde family target tyrosine residues for phosphoribosyl-linked ubiquitination. RSC Chem Biol. 2: 1509-1519.doi: 10.1039/d1cb00088h. Abstract

Mares CA, Lugo FP, Albataineh M, Goins BA, Newton IG, Isberg RR, Bergman MA 2021. Heightened virulence of Yersinia is associated with decreased function of the YopJ protein. Infect Immun. Sep 20:IAI0043021. doi: 10.1128/IAI.00430-21.. Abstract

Bai J, Dai Y, Farinha A, Tang AY, Syal S, Vargas-Cuebas G, van Opijnen T, Isberg RR, Geisinger E. 2021. Essential gene analysis in Acinetobacter baumannii by high-density transposon mutagenesis and CRISPR interference. J Bacteriol. 29:JB.00565-20. doi: 10.1128/JB.00565-20. Abstract

Geisinger E, Mortman NJ, Dai Y, Cokol M, Syal S, Farinha A, Fisher DG, Tang AY, Lazinski DW, Wood S, Anthony J, van Opijnen T, Isberg RR. 2020. Antibiotic susceptibility signatures identify potential antimicrobial targets in the Acinetobacter baumannii cell envelope. Nat Comm. 11: 4522. doi: 10.1038/s41467-020-18301-2. Abstract

Zhu Z, Surujon D, Ortiz-Marquez JC, Huo W, Isberg RR, Bento J, van Opijnen T. 2020. Entropy of a bacterial stress response is a generalizable predictor for fitness and antibiotic sensitivity. Nat Commun. 11: 4365. doi: 10.1038/s41467-020-18134-z. Abstract

Clark SA, Thibault D, Shull LM, Davis KM, Aunins E, van Opijnen T, Isberg R. 2020. Topologically correct synthetic reconstruction of pathogen social behavior found during Yersinia growth in deep tissue sites. Elife. 9: e58106. Abstract

Anand IS, Choi WY, Isberg RR. 2020. Components of the endocytic and recycling trafficking pathways interfere with the integrity of the Legionella-containing vacuole. Cll Microbiol. 22: e13151. Abstract

Anand I, Choi W, Isberg RR. 2020. The vacuole guard hypothesis: how intravacuolar pathogens fight to maintain the integrity of their beloved home. Curr Opin Microbiol. 54: 51-58. Abstract

Thibault D, Jensen PA, Wood S, Qabar C, Clark S, Shainheit MG, Isberg RR, van Opijnen T. 2019. Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes. Nat Commun. 10: 5729.. Abstract

Kjellin J, Pränting M, Bach F, Vaid R, Edelbroek B, Li Z, Hoeppner MP, Grabherr M, Isberg RR, Hagedorn M, Söderbom F. 2019. Investigation of the host transcriptional response to intracellular bacterial infection using Dictyostelium discoideum as a host model. BMC Genomics 20: 961. Abstract

Christenson ET, Isaac DT, Yoshida K, Lipo E, Kim JS, Ghirlando R, Isberg RR, Banerjee A 2019. The iron-regulated vacuolar Legionella pneumophila MavN protein is a transition-metal transporter. Proc Natl Acad Sci USA 116: 17775-17785. Abstract

Davis KM, Krupp J, Clark S, Isberg RR. 2019. Iron-sulfur cluster repair contributes to Y. pseudotuberculosis survival within deep tissues. Infect Immun. 87: e00533-19. Abstract

Geisinger E, Huo W, Hernandez-Bird J, Isberg RR. 2019. Acinetobacter baumannii: Envelope determinants that control drug resistance, virulence, and surface variability. Annu Rev Microbiol. 73: 481-506. Abstract

Geisinger E, Vargas-Cuebas G, Mortman NJ, Syal S, Dai Y, Wainwright EL, Lazinski D, Wood S, Zhu Z, Anthony J, van Opijnen T, Isberg RR. 2019. The landscape of phenotypic and transcriptional responses to diprofloxacin in Acinetobacter baumannii: Acquired resistance alleles modulate drug-induced SOS response and prophage replication. MBio 10: e01127-19. Abstract

Fasciano AC, Mecsas J, Isberg RR. 2019. New age strategies to reconstruct mucosal tissue colonization and growth in cell culture systems. Microbiol Spectr. 7. doi: 10.1128/microbiolspec.BAI-0013-2019. Abstract

Sol A, Lipo E, de Jesús-Díaz DA, Murphy C, Devereux M, Isberg RR. 2019. Legionella pneumophila translocated translation inhibitors are required for bacterial-induced host cell cycle arrest. Proc Natl Acad Sci USA. 116: 3221-3228. Abstract

Davis KM, Isberg RR. 2019. One for all, but not all for one: Social behavior during bacterial diseases. Trends Microbiol. 27 64-74. Abstract

Morgan JM, Lam HN, Delgado J, Luu J, Mohammadi S, Isberg RR, Wang H, Auerbuch V. 2018. An experimental pipeline for initial characterization of bacterial Type III secretion system inhibitor mode of action using enteropathogenic Yersinia. Front Cell Infect Microbiol. 8: 404. Abstract

Liu BC, Sarhan J, Panda A, Muendlein HI, Ilyukha V, Coers J, Yamamoto M, Isberg RR, Poltorak A. 2018. Constitutive interferon maintains GBP expression required for release of bacterial components upstream of pyroptosis and anti-DNA responses. Cell Rep. 24: 155-168. Abstract

Geisinger E, Mortman NJ, Vargas-Cuebas G, Tai AK, Isberg RR. 2018. A global regulatory system links virulence and antibiotic resistance to envelope homeostasis in Acinetobacter baumannii. PLoS Pathog. 14: e1007030. Abstract

de Jesús-Díaz DA, Murphy C, Sol A, Dorer M, Isberg RR. 2017. Host cell S phase restricts Legionella pneumophila intracellular replication by destabilizing the membrane-bound replication compartment. MBio 8: e02345-16. Abstract

Mitchell G, Isberg RR 2017. Innate immunity to intracellular pathogens: Balancing microbial elimination and inflammation. Cell Host Microboe 22: 166-175. Abstract

Geisinger E, Isberg RR. 2017. Interplay between antibiotic resistance and virulence during disease promoted by multidrug-resistant bacteria. J Infect. Dis. 215(suppl_1): S9-S17. Abstract

Kotewicz KM, Ramabhadran V, Sjoblom N, Vogel JP, Haenssler E, Zhang M, Behringer J, Scheck RA, Isberg RR. 2017. A single Legionella effector catalyzes a multistep ubiquitination pathway to rearrange tubular endoplasmic reticulum for replication. Cell Host Microbe 21: 169-181. Abstract

Sheehan KB, Martin M, Lesser CF, Isberg RR, Newton IL. 2016. Identification and characterization of a candidate Wolbachia pipientis type IV effector that interacts with the actin cytoskeleton. MBio 7: e00622-e00216. Abstract

Shen H, Gonzalez-Juarbe N, Blanchette K, Crimmins G, Bergman MA, Isberg RR, Orihuela CJ, Dube PH. 2016. CD8+ T cells specific to a single Yersinia pseudotuberculosis epitope restrict bacterial replication in the liver but fail to provide sterilizing immunity. Infect Genet Evol. 43:289-296. Abstract

Davis KM, Isberg RR. 2016. Defining heterogeneity within bacterial populations via single cell approaches. Bioessays 38: 782-790. Abstract

O'Connor TJ, Zheng H, VanRheenen SM, Ghosh S, Cianciotto NP, Isberg RR. 2016. Iron limitation triggers early egress by the intracellular bacterial pathogen Legionella pneumophila. Infect Immun. 84: 2185-2197.  Abstract

Hempstead AD, Isberg RR 2015. Inhibition of host cell translation elongation by Legionella pneumophila blocks the host cell unfolded protein response. Proc Natl Acad Sci USA 112: E6790-E6797.   Abstract

Chen Y, Lin Y, Davis KM, Wang Q, Rnjak-Kovacina J, Li C, Isberg RR, Kumamoto CA, Mecsas J, Kaplan DL 2015. Robust bioengineered 3D functional human intestinal epithelium. Sci Rep. 5: 13708.   Abstract

Isaac DT, Laguna RK, Valtz N, Isberg RR. 2015. MavN is a Legionella pneumophila vacuole-associated protein required for efficient iron acquisition during intracellular growth. Proc Natl Acad Sic USA 112: E5208-E5217.  Abstract

Haenssler E, Ramabhadran V, Murphy CS, Heidtman MI, Isberg RR. 2015. Endoplasmic reticulum tubule protein Reticulon 4 associates with the L. pneumophila vacuole and with translocated substrate Ceg9. Infect Immun. 83: 3479-3489.  Abstract

Asrat S, Davis KM, Isberg RR. 2015. Modulation of the host innate immune and inflammatory response by translocated bacterial proteins. Cell Microbiol. 17: 785-795. Abstract

Sheahan KL, Isberg RR. 2015.Identification of mammalian proteins that collaborate with type III secretion system function: Involvement of a chemokine receptor in supporting translocon activity. MBio 6: e02023-14. Abstract

Geisinger E, Isberg RR 2015. Antibiotic modulation of capsular exopolysaccharide and virulence in Acinetobacter baumannii. PLoS Pathog 11: e1004691. Abstract

Davis KM, Mohammadi S, Isberg RR. 2014. Community behavior and spatial regulation within a bacterial microcolony in deep tissue sites serves to protect against host attack. Cell Host Microbe 17: 21-31. Abstract

Davis KM, Isberg RR 2014. Plague's partners in crime. Immunity 41: 347-349. Abstract

Asrat S, de Jesús DA, Hempstead AD, Ramabhadran V, Isberg RR. 2014. Bacterial pathogen manipulation of host membrane trafficking. Ann Rev Cell Dev Biol. 30: 79-109. Abstract

Asrat S, Dugan AS, Isberg RR 2014. The frustrated host response to Legionella pneumophila Is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. PLoS Pathog. 10: e1004229. Abstract

O'Connor TJ, Isberg RR. 2014. iMAD, a genetic screening strategy for dissecting complex interactions between a pathogen and its host. Nat Protoc. 9: 1916-1930. Abstract

Isaac DT, Isberg R. 2014. Master manipulators: an update on Legionella pneumophila Icm/Dot translocated substrates and their host targets. Front Microbiol. 9: 343-359. Abstract

Creasey EA, Isberg RR. 2014. Maintenance of vacuole integrity by bacterial pathogens. Curr Opin Microbiol. 17C: 46-52. Abstract